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Trajectory and Functional Analysis of PD-1high CD4+CD8+ T Cells in Hepatocellular Carcinoma by Single-Cell Cytometry and Transcriptome Sequencing
Citation:
Zheng, B. et al. Trajectory and Functional Analysis of PD-1high CD4+CD8+ T Cells in Hepatocellular Carcinoma by Single-Cell Cytometry and Transcriptome Sequencing. Advanced Science 7, (2020).
Here, a single-cell study involving 17 432 600 immune cells of 39 matched HCC (T), nontumor (N), and leading-edge (L) specimens by mass cytometry is conducted.
Analysis plots of cells or proteins within dataset:
MDS plot showing similarities between samples in dataset. Colored by sample conditions.
Barplot showing the number of cells measured for each sample in the dataset. Bars are colored by experimental condition.
Clustering of samples (rows) and markers (columns) based on the median (arcsinh-transformed) marker expression across all cells measured for each sample. Colored by experimental condition. Dendrograms are based on hierarchical clustering with Euclidean distance metric and average linkage
Non-redundancy scores for markers and all samples in the dataset. The points represent the per-sample NR scores (colored by experimental conditions), while open white circles indicate the mean NR scores from all the samples. Markers on the x-axis are sorted according to the decreasing average NRS