In this study, we used high-parameter single-cell phenotyping by CyTOF to define the features of blood- and LN-derived T and NK cells from three groups of aviremic PLWH: elite controllers, and HIV+ ART-suppressed individuals who had initiated ART during the acute vs. chronic phases of infection. Our main findings are that T and NK cells from the acutely-treated individuals harbour unique phenotypic features, and differ markedly depending on whether they derived from the blood or LN compartment. Included are FCS files of CyTOF datasets for blood (“PBMC”) and lymph node (“LN”) specimens from three groups of aviremic individuals living witih HIV: elite controllers (“Ctrl”), ART-suppressed individuals who initiated treatment during chronic infection (“Chronic”), and ART-suppressed individuals who initiated treatment during acute infection (“Acute”). All data files are pregated on live, singlet events (“Singlets”). Patient IDs are indicated as four digit numbers.